Protein_Domain

Part:BBa_K2401012

Designed by: Shanti Ricke, Alina Nicolai   Group: iGEM17_Hamburg   (2017-10-21)


HMWP 1 High molecular weight synthase 1 expression system

This part synthesizes HMWP1 a non-ribosomal synthetase which completes the synthesis of yersiniabactin from a nor-pyochelin template.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1880
    Illegal NgoMIV site found at 3055
    Illegal NgoMIV site found at 3794
    Illegal NgoMIV site found at 4301
    Illegal NgoMIV site found at 9281
    Illegal AgeI site found at 979
    Illegal AgeI site found at 2467
    Illegal AgeI site found at 3425
    Illegal AgeI site found at 4342
    Illegal AgeI site found at 9581
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2006
    Illegal BsaI.rc site found at 10525
    Illegal SapI site found at 961
    Illegal SapI site found at 6778
    Illegal SapI.rc site found at 1406


[edit]
Categories
Parameters
None